Chemistry Department of the Indian Institute of Technology Bombay organized an Institute lecture series by our distinguished visiting Prof. G Krishnamoorthy between on April 18 and April 26, 2019, on the new central facility of time-resolved fluorescence established in SAIF CRNTS building.
FLIM uses fluorescence lifetime, rather than intensity, to generate images. Appropriate use of pseudo color gives a significantly better contrast. Besides, one can study fluorescence lifetimes in specific locations inside small volumes, e,g, the fluorescence lifetime of a marker dye in the cell membrane and near mitochondria. This can provide useful information about the properties of the microenvironment. FCS, on the other hand, provides diffusion coefficients rather easily. This technique has been used widely to study protein folding, fibrillation, etc. Examples of FLIM and FCS data recorded on our microscope are available at https://tinyurl.com/FLIM-FCS. Our microscope is from Picoquant, Germany. It is possible to perform single molecule/ single particle level studies in it.
About the speaker:
Prof. G Krishnamoorthy is currently Professor of Chemistry & Head of central and instruments facility at the Indian Institute of Guwahati. His area of research includes photochemistry and Fluorescence Spectroscopy. He is a Ph.D. from Indian Institute of Kanpur and has published a lot of research papers in his field. He also has been rewarded and recognized by the American Chemical Society for his contribution to submit an article for the special issue on Photoinduced Proton Transfer special issue and by Royal Chemical Society for submitting a review article for the special issue on Hydrogen Bonding in the Excited State.