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Human Brain Proteome Map

Name: Athithyan Paramasivan, Sanjyot Shenoy, Deeptarup Biswas, and Chetanya Gupta

Department: Chemistry

Name of supervisor: Prof. Sanjeeva Srivastava

 

Description of research work: 

The human brain is an organ of deep interest and intrigue. Due to its principal role in cognition and thinking, it has been a subject of intrigue throughout history. Presumably due to its complexity, there are still many questions left unanswered, and recent findings help fill the lacunae in small parts. The brain is divided into two hemispheres, each with different composition and hence different functionalities, but due to the difficulty in obtaining reliable samples, very less is known about the inter-hemispheric relationship and biological implications. In this study, using mass- spectrometry based proteomics, we have analysed 19 subregions of the brain and have consolidated our findings in the website: www.brainprot.org.

We have used MS-based strategies for protein identification and tools like MaxQuant, MetaboAnalyst, etc. in our study.

Importance of this project:

Brain Lateralization is an important field of neuroscience research. How the left and right hemispheres differ from each other in their structure and function is one of the important questions in which the current neuroscience research is focused on. A lot of research in this area has already taken place in terms of genomics and transcriptomics, but in terms of proteomics, research is still lagging behind. In this project we aim to shed new light on brain lateralization through the lens of proteomics.

Through our work, we hope to advance neuroscience research, as our website is the most comprehensive reference for brain proteomics data based on our data and publicly available repository data and our study has considered 19 sub regions, which is the largest for a study of its kind till date. This study is a part of Human Brain Proteome Project (HBPP), an initiative of Human Proteome Organization (HUPO).

Novelty of this project:

We have done MS based proteomics analysis of 19 neuro-anatomical regions(21 regions were

analysed, but 2 regions’ data was not taken forward due to its lesser confidence) for both the hemispheres. All these proteins were taken forward for further analyses and inter-regional protein expression was found and also, regional characterization was performed. All of this data is available publicly for use at www.brainprot.org, which is a scalable and a dynamic portal. The portal will be updated further in future with new and updated information. To ensure reliability of results, the protein datasets of all regions were cross-checked with pre-existing databases/repositories and literature and our data corroborates such pre-existing

 

Inter-hemispheric Brain Proteome Map Website:

 

Overview of the workflow: